Column bleed needs to be discriminated from other similar bleedings such as septum bleed, column contamination and fouling of detector or liner. The region is generally free of any distinct peaks. Comparison between bleeding and normal column chromatogramsĬolumn bleeding is characterized by a steady rise in the baseline about 30 degrees C before the upper permissible temperature limit and reaches a plateau around the upper limit. It is natural for all columns, irrespective of stationary phase film thickness, to bleed at high temperatures but excessive bleeding results in shortening of the useful lifespan of the columns.
Thick films columns are used to handle higher sample loads but at same time their bleed rates are higher. The level of bleed depends on the film thickness of the stationary phase layer on the adsorbent.
Column bleeding results from thermal breakdown of the stationary phase at temperatures close to the upper temperature limit of the column. IEC: 0 to 500 mL/min makeup in 0.1 stepsįor budget-conscious labs, iConnect modules offer the flexibility to start with a single-channel instrument, and expand to multi-injector or multi-detector configurations to accommodate new applications or increased throughput requirements.Reduction of column bleed requires first of all a clear understanding of the term.Makeup Gas: Nitrogen or 95% argon/5% methane.Radioactive Source: 370 MBq equal to 10 mCi, 63 Ni.
Linear Dynamic Range: Greater than 10 4 with lindane.The iConnect Electron Capture Detector features a miniaturized cell equipped with a purged, removable anode, designed to maximize robustness towards the matrix effect while guaranteeing fast peak detection and maximum sensitivity.
This unique capability can also enable continuous operation or dramatically reduce maintenance downtime - just replace dirty units with clean backups and be back to running samples again in a few minutes. In two minutes, without special training or tools, you can swap out these modules and reconfigure your instrument for a specific workflow or method. Thermo Scientific “instant connect” (iConnect) injectors and detectors redefine flexibility and usability in GC and GC-MS analyses.
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